38.4 Folate (categories)

Folate range (<45.3 vs >=45.3)

38.4.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	33.6502151	1	<0.0001	***
Folate_gp	0.0759394	1	0.8	ns
Study	0.5650209	1	0.5	ns
GA_samp	20.0130767	1	<0.0001	***
Folate_gp:Study	0.0676829	1	0.8	ns

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	5.342630	1	0.02	*
Folate_gp	2.298542	1	0.1	ns
Study	2.161174	1	0.1	ns
GA_samp	317.190802	1	<0.0001	***
Folate_gp:Study	1.573336	1	0.2	ns

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	29.5105258	1	<0.0001	***
Folate_gp	0.5252367	1	0.5	ns
Study	0.4103290	1	0.5	ns
GA_samp	94.1392050	1	<0.0001	***
Folate_gp:Study	0.1495187	1	0.7	ns

Insulin (uU/ml)

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp	1	Inf	5.245	5.245	0.02	*
3	Study	1	Inf	3.750	3.750	0.05	ns
4	GA_samp	1	Inf	0.920	0.920	0.3	ns
2	Folate_gp:Study	1	Inf	0.015	0.015	0.9	ns

HOMA-IR

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp	1	Inf	6.900	6.900	0.009	**
3	Study	1	Inf	7.383	7.383	0.007	**
4	GA_samp	1	Inf	0.449	0.449	0.5	ns
2	Folate_gp:Study	1	Inf	0.116	0.116	0.7	ns

Glucose (mmol/L)

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp	1	Inf	8.911	8.911	0.003	**
3	Study	1	Inf	27.555	27.555	<0.0001	***
4	GA_samp	1	Inf	0.478	0.478	0.5	ns
2	Folate_gp:Study	1	Inf	2.501	2.501	0.1	ns

38.4.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	15.44 (6.19, 38.40)	<0.0001
Folate_gp≥45.3	1.01 (0.82, 1.24)	0.93
StudySTOP	1.12 (0.82, 1.55)	0.48
GA_samp	1.13 (1.07, 1.20)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.74 (1.04, 2.87)	0.03
Folate_gp≥45.3	0.94 (0.83, 1.07)	0.37
StudySTOP	1.20 (1.00, 1.44)	0.05
GA_samp	1.33 (1.29, 1.38)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.16 (0.09, 0.32)	<0.0001
Folate_gp≥45.3	0.92 (0.78, 1.09)	0.33
StudySTOP	0.93 (0.73, 1.20)	0.59
GA_samp	1.22 (1.17, 1.27)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	18.50 (4.95, 69.20)	<0.0001
Folate_gp≥45.3	0.75 (0.57, 0.97)	0.03
StudySTOP	0.65 (0.41, 1.04)	0.07
GA_samp	1.04 (0.96, 1.13)	0.34

HOMA-IR

Var	Effect	P
(Intercept)	5.72 (1.39, 23.54)	0.02
Folate_gp≥45.3	0.70 (0.53, 0.93)	0.01
StudySTOP	0.52 (0.32, 0.85)	0.009
GA_samp	1.03 (0.94, 1.13)	0.50

Glucose (mmol/L)

Var	Effect	P
(Intercept)	6.89 (5.31, 8.95)	<0.0001
Folate_gp≥45.3	0.90 (0.85, 0.96)	0.002
StudySTOP	0.79 (0.73, 0.85)	<0.0001
GA_samp	0.99 (0.97, 1.01)	0.51

38.4.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	72.05286	15.20468	56.19899	47.21477	109.9574
STOP	80.95288	13.51384	24.25452	57.37005	114.2298

hPL (μg/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	60.17924	4.912917	121.14938	51.19825	70.73564
STOP	72.16384	3.267865	41.06058	65.85731	79.07429

GH2 (ng/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	1.835249	0.2048767	142.13560	1.471824	2.288412
STOP	1.714043	0.0983096	52.70028	1.527755	1.923047

Insulin (uU/ml)

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	26.11216	5.075430	Inf	17.83998	38.22004
STOP	17.05872	1.464529	Inf	14.41680	20.18477

HOMA-IR

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	6.954116	1.443253	Inf	4.630033	10.444791
STOP	3.609646	0.333788	Inf	3.011293	4.326893

Glucose (mmol/L)

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	5.960279	0.2147877	Inf	5.553826	6.396479
STOP	4.710949	0.0946799	Inf	4.528987	4.900222

38.4.4 Comparisons (Overall)

Hormone	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥45.3 / <45.3	1.01 (0.82, 1.25)	0.9
hPL (μg/ml)	≥45.3 / <45.3	0.94 (0.83, 1.07)	0.4
GH2 (ng/ml)	≥45.3 / <45.3	0.92 (0.78, 1.09)	0.3
Insulin (uU/ml)	≥45.3 / <45.3	0.75 (0.57, 0.97)	0.03
HOMA-IR	≥45.3 / <45.3	0.70 (0.53, 0.93)	0.01
Glucose (mmol/L)	≥45.3 / <45.3	0.90 (0.85, 0.96)	0.002

38.4.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.4.6 Plots