38.4 Folate (categories)

Folate range (<45.3 vs >=45.3)

38.4.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 33.6502151 1 <0.0001 ***
Folate_gp 0.0759394 1 0.8 ns
Study 0.5650209 1 0.5 ns
GA_samp 20.0130767 1 <0.0001 ***
Folate_gp:Study 0.0676829 1 0.8 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 5.342630 1 0.02 *
Folate_gp 2.298542 1 0.1 ns
Study 2.161174 1 0.1 ns
GA_samp 317.190802 1 <0.0001 ***
Folate_gp:Study 1.573336 1 0.2 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 29.5105258 1 <0.0001 ***
Folate_gp 0.5252367 1 0.5 ns
Study 0.4103290 1 0.5 ns
GA_samp 94.1392050 1 <0.0001 ***
Folate_gp:Study 0.1495187 1 0.7 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 5.245 5.245 0.02 *
3 Study 1 Inf 3.750 3.750 0.05 ns
4 GA_samp 1 Inf 0.920 0.920 0.3 ns
2 Folate_gp:Study 1 Inf 0.015 0.015 0.9 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 6.900 6.900 0.009 **
3 Study 1 Inf 7.383 7.383 0.007 **
4 GA_samp 1 Inf 0.449 0.449 0.5 ns
2 Folate_gp:Study 1 Inf 0.116 0.116 0.7 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 8.911 8.911 0.003 **
3 Study 1 Inf 27.555 27.555 <0.0001 ***
4 GA_samp 1 Inf 0.478 0.478 0.5 ns
2 Folate_gp:Study 1 Inf 2.501 2.501 0.1 ns

38.4.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.44 (6.19, 38.40) <0.0001
Folate_gp≥45.3 1.01 (0.82, 1.24) 0.93
StudySTOP 1.12 (0.82, 1.55) 0.48
GA_samp 1.13 (1.07, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.74 (1.04, 2.87) 0.03
Folate_gp≥45.3 0.94 (0.83, 1.07) 0.37
StudySTOP 1.20 (1.00, 1.44) 0.05
GA_samp 1.33 (1.29, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.16 (0.09, 0.32) <0.0001
Folate_gp≥45.3 0.92 (0.78, 1.09) 0.33
StudySTOP 0.93 (0.73, 1.20) 0.59
GA_samp 1.22 (1.17, 1.27) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 18.50 (4.95, 69.20) <0.0001
Folate_gp≥45.3 0.75 (0.57, 0.97) 0.03
StudySTOP 0.65 (0.41, 1.04) 0.07
GA_samp 1.04 (0.96, 1.13) 0.34
HOMA-IR
Var Effect P
(Intercept) 5.72 (1.39, 23.54) 0.02
Folate_gp≥45.3 0.70 (0.53, 0.93) 0.01
StudySTOP 0.52 (0.32, 0.85) 0.009
GA_samp 1.03 (0.94, 1.13) 0.50
Glucose (mmol/L)
Var Effect P
(Intercept) 6.89 (5.31, 8.95) <0.0001
Folate_gp≥45.3 0.90 (0.85, 0.96) 0.002
StudySTOP 0.79 (0.73, 0.85) <0.0001
GA_samp 0.99 (0.97, 1.01) 0.51

38.4.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 72.05286 15.20468 56.19899 47.21477 109.9574
STOP 80.95288 13.51384 24.25452 57.37005 114.2298
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 60.17924 4.912917 121.14938 51.19825 70.73564
STOP 72.16384 3.267865 41.06058 65.85731 79.07429
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.835249 0.2048767 142.13560 1.471824 2.288412
STOP 1.714043 0.0983096 52.70028 1.527755 1.923047
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 26.11216 5.075430 Inf 17.83998 38.22004
STOP 17.05872 1.464529 Inf 14.41680 20.18477
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.954116 1.443253 Inf 4.630033 10.444791
STOP 3.609646 0.333788 Inf 3.011293 4.326893
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.960279 0.2147877 Inf 5.553826 6.396479
STOP 4.710949 0.0946799 Inf 4.528987 4.900222

38.4.4 Comparisons (Overall)

Hormone Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥45.3 / <45.3 1.01 (0.82, 1.25) 0.9
hPL (μg/ml) ≥45.3 / <45.3 0.94 (0.83, 1.07) 0.4
GH2 (ng/ml) ≥45.3 / <45.3 0.92 (0.78, 1.09) 0.3
Insulin (uU/ml) ≥45.3 / <45.3 0.75 (0.57, 0.97) 0.03
HOMA-IR ≥45.3 / <45.3 0.70 (0.53, 0.93) 0.01
Glucose (mmol/L) ≥45.3 / <45.3 0.90 (0.85, 0.96) 0.002

38.4.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.4.6 Plots